GSM3020538: Eed KO Macrophage H3K27ac ChIP Rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Digestive tract
Cell type
Intestinal villus
NA
NA
Attributes by original data submitter
Sample
source_name
mouse intestinal villus cells
antibody
H3K27ac
tissue
mouse intestinal villus cells
strain
B6; 129 mixed background
cell type
Adult Epithelial cells; proximal 1/3 of intestine
developmental stage
adult
genotype
Eed F/F; RosaFlox-STOP-Flox-EYFP
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
C-Kit+ cells were purified from mouse bone marrow using CD117 microbead and cultured. Cells were harvested, snap-frozen in liquid nitrogen, and used for subsequent ChIP analysis. Whole cell lysates were sonicated and incubated with H3K27ac antibody overnight (Active Motif 39135) for immunoprecipitation. ThruPLEX DNA-seq Kit (Rubicon Genomics) was used to prepare libraries, and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.