Antigen

Antigen Class

Histone

Antigen

H3K36me2

Cell type

Cell type Class

Muscle

Cell type

C2C12

Primary Tissue

Skeletal Muscle

Tissue Diagnosis

NOS

Sample

source_name

C2C12

treatment 1

DMEM containing 2% donor horse serum (EuroClone)_ 24 hours (day1)

chip antibody

Rabbit anti-dimethyl-Histone H3 (Lys 36) (#07-369; Millipore)

cell type

myoblasts

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For DNA purification, the QIAquick PCR purification kit was used (Qiagen). DNA was further sonicated after elution, using the Ultra-Sonicator E220 (CovarisTM) at 10 V for 90 seconds, and the fragments sizes were evaluated by the Bioanalyzer (Agilent Technologies) Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

mm10

Number of total reads

24747490

Reads aligned (%)

96.7

Duplicates removed (%)

51.0

Number of peaks

92 (qval < 1E-05)

mm9

Number of total reads

24747490

Reads aligned (%)

96.7

Duplicates removed (%)

50.9

Number of peaks

72 (qval < 1E-05)