ChIP-Atlas: SRX3729177

Antigen

Cell type Class: Liver
Cell type: Liver
MeSH Description: A large lobed glandular organ in the abdomen of vertebrates that is responsible for detoxification, metabolism, synthesis and storage of various substances.

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: Chromatin was extracted from frozen liver tissue from each of 4 individual livers per group (exception: n=3 for DKO Female_H3K4me1). Crosslinking was achieved by adding formaldehyde to a final concentration of 1% for 5 min at room temperature. Samples were sonicated to a 100-300 bp size range. ChIP was performed using the following ChIP-validated antibodies: H3K27ac (Abcam Cat # ab4729, 3 μg antibody per 15 μg of sonicated chromatin), H3K4me1 (Abcam Cat # ab8895, 1.2 μg antibody per 15 μg of sonicated chromatin), H3K27me3 (Abcam Cat # ab6002, 2 μg antibody per 10 μg of sonicated chromatin), and normal rabbit IgG (Santa Cruz, Cat # sc-2027, 3 μg antibody per 15 μg of sonicated chromatin). Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep kit for Illumina (NEB, catalog no. E7645) according to the manufacturer's instructions. NEBNext Multiplex Oligos for Illumina (NEB, Set 1; catalog no. E7335, NEB, Set 2; catalog no. E7500) were used for multiplexing.