Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Larvae

Cell type

3rd instar brain

NA

NA

Sample

source_name

L3 Brains

developmental stage

L3

tissue

brains

chip antibody

none

replicates

2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

400 L3 larvae of the ato:GFP genotype were dissect to isolate 400 brains that were later fixed and sonicated. A 10% of the sonicated volume was used as the input. The rest was used to isolate the DNA bound to the transcription factor Atonal using anti GFP antibodies. TruSeqLT

Sequencing Platform

instrument_model

NextSeq 500

dm6

Number of total reads

51245602

Reads aligned (%)

90.4

Duplicates removed (%)

30.1

Number of peaks

3778 (qval < 1E-05)

dm3

Number of total reads

51245602

Reads aligned (%)

90.9

Duplicates removed (%)

26.9

Number of peaks

0 (qval < 1E-05)