Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pancreas

Cell type

Islets of Langerhans

MeSH Description

Irregular microscopic structures consisting of cords of endocrine cells that are scattered throughout the PANCREAS among the exocrine acini. Each islet is surrounded by connective tissue fibers and penetrated by a network of capillaries. There are four major cell types. The most abundant beta cells (50-80%) secrete INSULIN. Alpha cells (5-20%) secrete GLUCAGON. PP cells (10-35%) secrete PANCREATIC POLYPEPTIDE. Delta cells (~5%) secrete SOMATOSTATIN.

Sample

source_name

pancreatic islets

treatment

IL1B 10 d

strain

C57BL/6NRj

gender

male

age

12 weeks

tissue

pancreatic islets

chip antibody

H3K4me3 (Cat#04-745 Milipore)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared using KAPA DNA Library Preparation Kits for Illumina.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

30051544

Reads aligned (%)

83.3

Duplicates removed (%)

48.5

Number of peaks

22540 (qval < 1E-05)

mm9

Number of total reads

30051544

Reads aligned (%)

83.2

Duplicates removed (%)

48.4

Number of peaks

22545 (qval < 1E-05)