SRX3709366
GSM3011688: Abf1 ChIPmentation (alt ChIP-seq); Saccharomyces cerevisiae; ChIP-Seq

Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ABF1

Cell type

Cell type Class
Yeast strain
Cell type
BY4741
NA
NA

Attributes by original data submitter

Sample

source_name
yeast
chip antibody
Abf1-TAP (Sigma i5006-100MG, Lot#SLBM2617V)
cell description
GRF88, MATa his4-38, S288C background
strain
BY4741

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with IgG antibody. Libraries were prepared for sequencing using standard Illumina protocols with modifications; Reference paper:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813302/

Sequencing Platform

instrument_model
NextSeq 500

Where can I get the processing logs?

Read processing pipeline log

sacCer3

Number of total reads
2467996
Reads aligned (%)
77.4
Duplicates removed (%)
14.9
Number of peaks
495 (qval < 1E-05)

Base call quality data from DBCLS SRA