GSM3003482: ATACseq CAID 3 p8; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Epidermis
Cell type
Dermal fibroblast
NA
NA
Attributes by original data submitter
Sample
source_name
Human dermal fibroblasts
cell type
Dermal fibroblasts
genotype
SGO1-/-
passage
8
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were lysed with ice-cold hypotonic cell lysis buffer (0.1% w/v Sodium Citrate Tribasic Dihydrate and 0.1% v/v Triton X-100) for 30 min on ice followed by a second 30 min lysis step with ice-cold cell lysis buffer (10 mM Tris-HCl, pH 7.4, 10 mM NaCl, 3 mM MgCl 2, 0.1% v/v IGEPAL CA-630). Transposition reaction was carried out in 25 μl reaction volume using 5 μl of tagmentation DNA enzyme in 2x TD buffer (FC-121-1030, Illumina). EDTA (25mM) was added to the reaction mix before DNA recovery with MiniElute PCR Purification columns (28004, Qiagen). ATAC-seq libraries were generated from 100,000 cells, as previously described (Buenrostro et al., 2013)