Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

HL-60

Primary Tissue

Blood

Tissue Diagnosis

Leukemia

Sample

source_name

HL-60 cell

cell type

Human promyelocytic leukemia cells

treatment

32 nM TPA

passages

80-85

chip antibody

RNA Pol II (Santa Cruz [sc-899 X])

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The construction of library was performed using TruSeq ChIP Library Preparation Kits according to the manufacturer's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

7991273

Reads aligned (%)

91.2

Duplicates removed (%)

6.2

Number of peaks

920 (qval < 1E-05)

hg19

Number of total reads

7991273

Reads aligned (%)

90.1

Duplicates removed (%)

6.3

Number of peaks

902 (qval < 1E-05)