GSM3001099: RNA PolII ChIP-seq, DMSO-treated HL-60 cells; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
RNA polymerase
Antigen
RNA polymerase II
Cell type
Cell type Class
Blood
Cell type
HL-60
Primary Tissue
Blood
Tissue Diagnosis
Leukemia
Attributes by original data submitter
Sample
source_name
HL-60 cell
cell type
Human promyelocytic leukemia cells
treatment
DMSO
passages
80-85
chip antibody
RNA Pol II (Santa Cruz [sc-899 X])
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. The construction of library was performed using TruSeq ChIP Library Preparation Kits according to the manufacturer's instructions.