GSM2991156: U87 scramble H3K27ac; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K27ac
Cell type
Cell type Class
Neural
Cell type
U-87 MG
Primary Tissue
Brain
Tissue Diagnosis
Glioblastoma
Attributes by original data submitter
Sample
source_name
U87_scramble_H3K27ac
cell line
U87
cell type
glioblastoma cells
transfected with
scramble control
chip antibody
H3K27ac (Abcam, Cat#ab45173, Lot GR276932-1)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Construction of ChIP-seq library was completed according to Illumina's instructions accompanying the NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs #E7370)