Antigen

Antigen Class

Histone

Antigen

H2A.Z

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

Embryonic fibroblasts

cell type

Embryonic fibroblasts

passages

5-10 passages

genotype

ANP32E KO

chip antibody

H2AZ

genetic background

C57BL/6

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions. The libraries were sequenced on the Illumina Hiseq 2500 as single-end 50 base reads following Illumina's instructions.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

60799182

Reads aligned (%)

98.2

Duplicates removed (%)

14.5

Number of peaks

34438 (qval < 1E-05)

mm9

Number of total reads

60799182

Reads aligned (%)

98.0

Duplicates removed (%)

14.5

Number of peaks

34479 (qval < 1E-05)