Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Epitope tags

Cell type

Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA

Attributes by original data submitter

Sample

source_name
mES cells
cell type
mES cells
treatment
DRB
genotype/variation
Fbio-tagged WDR43
antibody
FLAG, Sigma F1804

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total RNA of RNA-Seq were extracted by TRIzol reagent according to manufacturer's instruction. mRNA were further isolated by Dynabeads mRNA purification kit (Life Technologies) according to manufacturer's instruction. Libraries were prepared by NEBNEXT ChIP-seq library preparation kit or NEBNEXT Ultra RNA-seq library preparation kit RNA-seq library preparation kit

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
23481050
Reads aligned (%)
97.2
Duplicates removed (%)
29.8
Number of peaks
630 (qval < 1E-05)

mm9

Number of total reads
23481050
Reads aligned (%)
97.1
Duplicates removed (%)
29.9
Number of peaks
632 (qval < 1E-05)

Base call quality data from DBCLS SRA