GSM2987414: HepG2 shCtrl input-rep2 (ChIP-seq); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Liver
Cell type
Hep G2
Primary Tissue
Liver
Tissue Diagnosis
Carcinoma Hepatocellular
Attributes by original data submitter
Sample
source_name
HepG2 cells
cell line
HepG2
shRNA
Non-specific control
genotype
control
chip antibody
none
replicate
2
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cell lysate was sonicated and clarified by using 0.22 µm filter. Histone-DNA complexes were isolated with H3K36me3 antibody. Libraries were constructed by using Auto iDeal ChIP-seq kit for Histones (Diagenode, C01010057) following manufacturer's prorocol.