Antigen

Antigen Class

TFs and others

Antigen

NKX2-1

Cell type

Cell type Class

Lung

Cell type

NCI-H441

Primary Tissue

Lung

Tissue Diagnosis

Adenocarcinoma Papillary

Sample

source_name

H441_TTF-1_0h

ttf-1 expression

positive

cell line

H441

histology

lung adenocarcinoma

chip antibody

TTF-1 [Thermo Scientific, Catalog#: MS-699-P clone 8G7G3/1]

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP were performed as described (Koinuma et al, Mol Cell Biol 29: 172-186, 2009). SMAD3, SMAD4, or TTF-1-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model

Illumina Genome Analyzer IIx

hg19

Number of total reads

33781909

Reads aligned (%)

86.5

Duplicates removed (%)

6.4

Number of peaks

59962 (qval < 1E-05)

hg38

Number of total reads

33781909

Reads aligned (%)

88.0

Duplicates removed (%)

5.4

Number of peaks

60169 (qval < 1E-05)