Approximately 2x10^7 N2A cells per ChIP were double cross-linked, first using 2 mM disuccinimidyl glutarate (DSG) for 45 min at room temperature, followed by 1% formaldehyde for 15 min at room temperature. 5 ug anti-KAP1 (ab10483, Abcam) antibody was used for IP. Chromatin was sheared to approximately 200 bp using a Bioruptor Pico (Diagenode) and ChIP DNA and matched input DNA from two independent KAP1 ChIP experiments were sequenced on an Illumina HiSeq 4000 (150 bp paired-end sequencing).