Antigen

Antigen Class

TFs and others

Antigen

PBX1

Cell type

Cell type Class

Blood

Cell type

Natural Killer T-Cells

MeSH Description

A specific natural killer T-cell subtype that expresses an invariant T-cell receptor alpha-chain.

Sample

source_name

Decidua NK cells

tissue

decidua tissue

cell type

CD56+CD3- NK cells

antibody

Rabbit anti-human PBX1 (CST, Cat# 4342S, RRID:AB_2160295)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and transcription factor PBX1-DNA complexes were isolated with antibody. A total amount of 10ng qualified ChIP-ed DNA per sample was used as input material for the library preparation. The sequencing libraries were generated using the VAHTS Universal DNA Library Prep Kit for Illumina® (Vazyme, ND604) following manufacturer's recommendations. Firstly, the end repair of DNA fragments was performed and an "A" base was added at the 3'-end of each strand. Adapters (Vazyme, N801) were then ligated to both ends of the end repaired/dA tailed DNA fragments for amplification and sequencing. The DNA fragments were purified and selected with VAHTSTM DNA Clean Beads (Vazyme, N411) in order to get appropriate size for sequencing. PCR was performed and aimed products were purified finally.

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

23955806

Reads aligned (%)

96.1

Duplicates removed (%)

17.5

Number of peaks

1001 (qval < 1E-05)

hg19

Number of total reads

23955806

Reads aligned (%)

94.9

Duplicates removed (%)

17.8

Number of peaks

315 (qval < 1E-05)