Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Foxl2

Cell type

Cell type Class
Embryo
Cell type
Embryonic ovaries
NA
NA

Attributes by original data submitter

Sample

source_name
E14.5 XX gonads
strain
CD1
tissue
ovaries
age
E14.5
antibody
FOXL2 (Novus, NB100-1277)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Ovaries from E14.5 CD1 embryos were separated from the mesonephros, snap-frozen, and stored at -80°C. Two independent ChIP-seq experiments were performed by Active Motif Inc. using 20-30 ug of shared chromatin from different pools of embryonic ovaries (n=80-100 ovaries/ChIP), and 4 ug of FOXL2 antibody (Novus, NB100-1277). Libraries were prepared by Active Motif Inc.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
28614005
Reads aligned (%)
95.7
Duplicates removed (%)
18.4
Number of peaks
7021 (qval < 1E-05)

mm9

Number of total reads
28614005
Reads aligned (%)
95.6
Duplicates removed (%)
18.5
Number of peaks
7017 (qval < 1E-05)

Base call quality data from DBCLS SRA