Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Epidermis

Cell type

BJ

Primary Tissue

Skin

Tissue Diagnosis

Normal

Sample

source_name

BJ Fibroblasts

cell line background

BJ-5ta

cell type

immortalized BJ foreskin fibroblast cell line

genotype/variation

FOXA2 doxycycline inducible

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Tagmentation was performed on whole nuclei at 37C for 45 minutes as previously described in (Buenrostro et al., 2013). DNA was isolated on PCR min-elute columns (Qiagen) and a small amount of the DNA was amplified for 9, 12 and 15 cycles to determine optimal cycling conditions. Nextera amplified

Sequencing Platform

instrument_model

Illumina MiSeq

hg38

Number of total reads

16324559

Reads aligned (%)

99.5

Duplicates removed (%)

0.9

Number of peaks

31243 (qval < 1E-05)

hg19

Number of total reads

16324559

Reads aligned (%)

99.0

Duplicates removed (%)

0.8

Number of peaks

31201 (qval < 1E-05)