Antigen

Antigen Class

TFs and others

Antigen

SMAD3

Cell type

Cell type Class

Digestive tract

Cell type

SW 480

Primary Tissue

unresolved

Tissue Diagnosis

unresolved

Sample

source_name

colon cancer cells

cell type

colon cancer cells

cell line

SW480

passage

5-10

antibody

SMAD3 ( Abcam, catalog: ab28379, lot: GR321957-3)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were sonicated and the SMAD3-DNA complexes were isolated via immunoprecipitation with a SMAD3 antibody. Illumina's DNA smart ChIP-seq

Sequencing Platform

instrument_model

HiSeq X Ten

hg38

Number of total reads

22185326

Reads aligned (%)

96.7

Duplicates removed (%)

15.2

Number of peaks

883 (qval < 1E-05)

hg19

Number of total reads

22185326

Reads aligned (%)

95.5

Duplicates removed (%)

15.5

Number of peaks

489 (qval < 1E-05)