Antigen

Antigen Class

ATAC-Seq

Antigen

ATAC-Seq

Cell type

Cell type Class

Blood

Cell type

ALL xenograft

NA

NA

Sample

source_name

ALL xenograft cells

tissue

ALL xenograft cells

xenograft id

ALL50

treatment

vehicle

Sequenced DNA Library

library_strategy

ATAC-seq

library_source

GENOMIC

library_selection

other

library_construction_protocol

Cell suspensions of spleens were prepared and mononuclear cells enriched to >97% human by density gradient centrifugation. The spleen-harvested cells were used for ATAC-seq assay. Buenrostro, J.D., Wu, B., Chang, H.Y. & Greenleaf, W.J. ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide. Curr Protoc Mol Biol 109, 21 29 21-29 (2015).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

hg38

Number of total reads

64390623

Reads aligned (%)

82.5

Duplicates removed (%)

31.8

Number of peaks

13136 (qval < 1E-05)

hg19

Number of total reads

64390623

Reads aligned (%)

82.0

Duplicates removed (%)

32.9

Number of peaks

12257 (qval < 1E-05)