GSM2971206: exp1 Control H3K4me1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me1
Cell type
Cell type Class
Muscle
Cell type
C2C12
Primary Tissue
Skeletal Muscle
Tissue Diagnosis
NOS
Attributes by original data submitter
Sample
source_name
differentiating C2C12 cells
cell type
differentiating myoblasts
antibody
Anti-mono-methylated histone H3K4 (Abcam: ab8895)
drug
vehicle
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were crosslinked with formaldehyde. Cell lysates were subjected to two-step DNA fragmentation by a probe-type sonifier and a water bath sonifier, and chromatin-DNA complexes were pulled down using the specific antibody. Libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) according to the manufacturer's instructions. Briefly, ChIP DNA was end-repaired using an end prep enzyme. After adapter ligation, DNA was PCR amplified with NEB Index primers for 8-10 cycles. The purified DNA was checked for quality on Agilent TapeStation instrument with an Agilent High Sensitivity D1000 kit. The library DNA was approximately 170–500 bp in length. Libraries ware quantified using quantitative real-time PCR with the Kapa Library Quantification Kit. Diluted library DNA was subjected NextSeq sequencers (Illumina) according to the manufacturer's protocols.