Curated Sample Data


Genome
dm3
Antigen Class
Histone
Antigen
H4K16ac
Cell type Class
Larvae
Cell type
3rd instar

Cell type information


NA
NA

Attributes by Original Data Submitter


source_name
third instar larvae
strain
msl-2-delta-7/10 / CyO-GFP
chip antibody
H4K16ac(Merck Milipore, 07-329)

Metadata from Sequence Read Archive

Library Description


library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Larvae were dissected and the front part fixed at RT for 15 min with 1% or 0.2% Formaldehyde. Chromatin was extracted using a sucrose cushion followed by Micrococcal Nuclease treatment and 10 cycles of sonication in a Bioruptor Pico. The lysate was clarified by centrifugation before immunoprecipitation, washes, elution by reverse crosslinking at 65deg for 14h. After RNaseA and ProteinaseK treatment, the DNA from Input and Immunoprecipitation was purified using Phenol-Chloroform Extraction and Ethanol precipitation. NEB Next Ultra II

Platform Information


instrument_model
Illumina HiSeq 3000

External Database Query

Logs in read processing pipeline


Number of total reads
16981582
Reads aligned (%)
98.0
Duplicates removed (%)
5.1
Number of peaks
4015 (qval < 1E-05)

Sequence Quality Data from DBCLS SRA