GSM2973392: Ishi E2 ATAC Seq; Homo sapiens; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Uterus
Cell type
Ishikawa
Primary Tissue
Uterus
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Ishikawa
cell line
Ishikawa
treatment
10 nM Estradiol 1 Hr
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Treated cells were trypsinized and purified by centrifugation before being place in nuclei isolation buffer (lysis buffer) containing 0.1% IGEPAL. Nuclei were then counted and 250,000 cells were used in ATAC-seq library preparation Libraries were constructed as described in ATAC-seq: A Method for Assaying Chromatin Accessibility Genome-Wide by Buenrostro et al. 2015 Curr Proc Mol Biol