Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Pluripotent stem cell

Cell type

ES cells

NA

NA

Sample

source_name

H3K4me3_1M-sonication-ChIP-ESC

strain

C57BL/6

cell type

cultured mESC

fixed by

formaldehyde for 3 min

chip antibody

H3K4me3, Millipore, 04-745, Lot: 2872328, 1.5 μg added

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Crosslinked cells were lysed and sonciated to 200-800 bp fragments. Soluble chromatin was used for ChIP experiments. DNA on beads were tagmented with Tn5 transposome following the Nextera protocol. After PCR enrichment using NEBNext Q5 Ultra II master mix, products were purified and size selected by AMPure XP beads for 200-1000 bp.

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

53631168

Reads aligned (%)

81.9

Duplicates removed (%)

11.1

Number of peaks

34196 (qval < 1E-05)

mm9

Number of total reads

53631168

Reads aligned (%)

81.8

Duplicates removed (%)

11.1

Number of peaks

34143 (qval < 1E-05)