Starting with approximately 1x10^8 C2C12 cells (crosslinked with formaldehyde), the chromatin immunoprecipitation was performed according to the Agilent Mammalian ChIP-on-chip Protocol version 10.0 using magnetic beads (Dynabeads® Protein G, Invitrogen), which were previously coated with monoclonal anti-flag antibodies (Monoclonal ANTI-FLAG® M2 Antibody, Sigma). ChIP DNA libraries were prepared for sequencing using standard Illumina protocols