Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

H3K4me3_HUVEC_siERG+siFLI1

cell type

HUVEC

stimulation

siERG+siFLI1, day7

chip antibody

H3K4me3 (MBL, MABI0304)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell lysate was sonicated and subsequently incubated with the antibody to isolate DNA. Libraries were prepared using KAPA Hyper Prep Kit (for illumina) (KAPA)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

33247456

Reads aligned (%)

81.0

Duplicates removed (%)

24.1

Number of peaks

18097 (qval < 1E-05)

hg19

Number of total reads

33247456

Reads aligned (%)

80.8

Duplicates removed (%)

24.3

Number of peaks

18028 (qval < 1E-05)