Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Cardiovascular

Cell type

HUVEC

Primary Tissue

Umbilical Cord

Tissue Diagnosis

Normal

Sample

source_name

H3K4me3_HUVEC_siControl

cell type

HUVEC

stimulation

siControl, day7

chip antibody

H3K4me3 (MBL, MABI0304)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Whole cell lysate was sonicated and subsequently incubated with the antibody to isolate DNA. Libraries were prepared using KAPA Hyper Prep Kit (for illumina) (KAPA)

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

26057957

Reads aligned (%)

50.1

Duplicates removed (%)

17.7

Number of peaks

14985 (qval < 1E-05)

hg19

Number of total reads

26057957

Reads aligned (%)

49.9

Duplicates removed (%)

18.1

Number of peaks

14966 (qval < 1E-05)