Antigen

Antigen Class

TFs and others

Antigen

SUZ12

Cell type

Cell type Class

Blood

Cell type

KG-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

KG1 cell line

cell line

KG1

tissue

bone marrow

culture properties

suspension

genotype

wild type

antibody

Suz12

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA or proteins-DNA complexes were isolated with antibody. DNA was purified using the i-Pure kit (Diagenode) Libraries were prepared according to Illumina's instructions accompanying the Illumina's TruSeq ChIP sample Prep Kit Protocol

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

46535515

Reads aligned (%)

97.3

Duplicates removed (%)

12.3

Number of peaks

1726 (qval < 1E-05)

hg19

Number of total reads

46535515

Reads aligned (%)

96.4

Duplicates removed (%)

13.7

Number of peaks

1312 (qval < 1E-05)