Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
EZH2

Cell type

Cell type Class
Blood
Cell type
KG-1
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
KG1 cell line
cell line
KG1
tissue
bone marrow
culture properties
suspension
genotype
shPLZF
antibody
EZH2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and histone-DNA or proteins-DNA complexes were isolated with antibody. DNA was purified using the i-Pure kit (Diagenode) Libraries were prepared according to Illumina's instructions accompanying the Illumina's TruSeq ChIP sample Prep Kit Protocol

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
84478936
Reads aligned (%)
89.0
Duplicates removed (%)
6.9
Number of peaks
2603 (qval < 1E-05)

hg38

Number of total reads
84478936
Reads aligned (%)
91.2
Duplicates removed (%)
5.4
Number of peaks
3177 (qval < 1E-05)

Base call quality data from DBCLS SRA