ChIP-Atlas: SRX3596421

Antigen

Cell type Class: Bone
Cell type: U2OS
Tissue: bone
Lineage: mesoderm
Description: osteosarcoma from the tibia of a 15 year old, J. Ponten and E. Saksela derived this line (originally 2T) in 1964 from a moderately differentiated sarcoma, viruses were not detected during co-cultivation with WI-38 cells or in CF tests against SV40, RSV or adenoviruses, mycoplasma contamination was detected and eliminated in 1972, (PMID: 6081590)

library_strategy: ATAC-seq
library_source: GENOMIC
library_selection: other
library_construction_protocol: U2OS-GRα cells grown on 6-well dishes were transfected with the appropriate siRNAs. After 48 h, the cells were treated with 100 nM dex or equivalent amounts of ethanol for 1 h before harvesting 50,000 cells and treating with transposase (FC-121-1030; Illumina). Transposition reaction was performed at 37 °C for 30 min, and the samples were purified using a Qiagen PCR MinElute kit (28006; Qiagen). Library fragments were amplified using Nextera PCR Primers (FC-121-1011; Illumina) and NEBnext PCR master mix (0541; New England Lab) for a total of 6–8 cycles. The libraries were then purified and size selected in the 100–450 bp range using Agencourt AMPure XP. Quality of the size-selected libraries were assessed using Agilent Technologies 2100 Bioanalyzer.