GSM2946599: HA-GLIS3 ChIP-Seq Dox Plus, 12hr; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Epitope tags
Cell type
Cell type Class
Pluripotent stem cell
Cell type
hESC H9
NA
NA
Attributes by original data submitter
Sample
source_name
G3-hESCs
treatment
Doxycycline Plus
cell line
H9 hESCs with Dox inducible Glis3 expression
time
12 hr
chip antibody
HA antibody (Cell Signaling, Catalog # C29F4)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-Seq, Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with A/G-conjugated magnetic beads with a HA-antibody. ChIPed DNA was amplified using a TruSeq ChIP Library prep kit.