Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CREBBP

Cell type

Cell type Class
Kidney
Cell type
293
Primary Tissue
Kidney
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
HEK293
cell line
Human Kidney Embryonic Cell line HEK293
genotype/variation
HSP70K71E-EGFP
chip antibody
anti-CBP [CBP/anti-KAT3A-chip grade, cat no. ab2832 ABCAM]

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP'd DNA was extracted accordingly in previous publication (Byun et al, ELL faciliates RNA polymerase II pause site entry and release. Nature Communications 3, 633 2012. ChIP-Seq samples were sequenced on GAIIx with 36bp length using Illumina TruSeq v3 Chemistry, Allthe samples have good quality with over 93% of bases having Q30 or above. Library complexity is measured by uniquely aligned reads using picard's markduplicate utility.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

hg19

Number of total reads
27523106
Reads aligned (%)
80.5
Duplicates removed (%)
13.4
Number of peaks
24020 (qval < 1E-05)

hg38

Number of total reads
27523106
Reads aligned (%)
81.9
Duplicates removed (%)
12.4
Number of peaks
22635 (qval < 1E-05)

Base call quality data from DBCLS SRA