GSM2942291: SA2 MCF10A ChIP-seq siSA1 Rep 1; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
STAG2
Cell type
Cell type Class
Breast
Cell type
MCF 10A
Primary Tissue
Breast
Tissue Diagnosis
Fibrocystic Disease
Attributes by original data submitter
Sample
source_name
Human Mammary Epithelial Cells
cell line
MCF10A
cell type
Transformed nontumorigenic
experiment
ChIP-SEQ
treatment
STAG1 siRNAs
chip antibody
custom made antibody for SA2 (STAG2)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
IP step of the experiment was performed adding 20% of mouse chromatin to human chromatin (600ug)- 720ug in total per replicate. 72Ug of antibody was used per replicate. Samples went through subsequent enzymatic treatments of end-repair, dA-tailing, and ligation to adapters with "NEBNext Ultra II DNA Library Prep Kit for Illumina" from New England BioLabs (catalog # E7645). Adapter-ligated library was completed by limited-cycle PCR and extracted with a [single] double-sided SPRI size selection. Median fragment size is 360 bp from which 120 bp correspond to adaptor sequences. Library was applied to an Illumina flow cell for cluster generation and sequenced on an Illumina instrument (see below) by following manufacturer's protocols.