GSM2936711: Input of ChIPseq for HP1a in Cbx1-/- mES cells, replicate 2; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Cbx5
Cell type
Cell type Class
Pluripotent stem cell
Cell type
ES cells
NA
NA
Attributes by original data submitter
Sample
source_name
Input of ChIPseq for HP1a in Cbx1-/- mES cells
strain background
hybrid 129-C57BL/6
genotype/variation
Cbx1 KO, Cbx3 flox
cell type
embryonic stem (ES) cells
treatment
no
antibody
Bio ChIP, Strepdavidin Dynabeads (Thermofisher)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
10^7 mES cells were crosslinked, chromatin was extracted and sonicated to an average size of 300bp. 1% of chromatin was set aside as Input control. The chromatin was incubated with Strapdavidin coupled Dynabeads, washed and de-crosslinked. Purified DNA was subsequently used for library preparation. For a detailed protocol, see Ostapcuk et al, 2017. Libraries were prepared using the NEB Next DNA library prep kit according to manufacturers instructions. For barcodes, see sample characteristics.