Antigen

Antigen Class

Histone

Antigen

H3K4me1

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7L Tamoxifen Resistant

cell types

immortalized breast epithelial cell line

chip antibody

H3K4me1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA extracted using Quick-RNA MiniPrep Kit from ZYMO Research TCC was performed as described (Kalhor et al. 2012). ChIP-seq and RNA-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

128994756

Reads aligned (%)

98.6

Duplicates removed (%)

6.9

Number of peaks

17410 (qval < 1E-05)

hg19

Number of total reads

128994756

Reads aligned (%)

97.9

Duplicates removed (%)

8.2

Number of peaks

17651 (qval < 1E-05)