Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7L parental

cell types

immortalized breast epithelial cell line

chip antibody

H3K4me3

agent

treated with E2

time point

1 hour

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

RNA extracted using Quick-RNA MiniPrep Kit from ZYMO Research TCC was performed as described (Kalhor et al. 2012). ChIP-seq and RNA-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

52764443

Reads aligned (%)

98.0

Duplicates removed (%)

6.3

Number of peaks

22334 (qval < 1E-05)

hg19

Number of total reads

52764443

Reads aligned (%)

97.0

Duplicates removed (%)

8.2

Number of peaks

21687 (qval < 1E-05)