Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K27ac

Cell type

Cell type Class
Blood
Cell type
MLL-AF9 leukemic cell
NA
NA

Attributes by original data submitter

Sample

source_name
mouse MLL-AF9 leukemia
cell line
MLL-AF9
cell type
leukemia cells
genotype
Setd1a knockout
chip antibody
H3K27Ac (abcam Cat#ab4729)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 2mM DSG for 30 min followed by 1% formaldehyde for 10 min and treated with 0.125M Glycine for 5 min. Washed cells were suspended in ChIP Lysis Buffer and shredded using Bioruptor (Diagenode) or E220 Focused-ultrasonicator (Covaris). Histone-DNA complexes were isolated with antibody. ChIP-seq libraries were constructed with the ThruPLEX DNA-seq Kit following the manufacturer's recommended protocol (Rubicon genomics).

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
36950668
Reads aligned (%)
93.3
Duplicates removed (%)
10.5
Number of peaks
29231 (qval < 1E-05)

mm9

Number of total reads
36950668
Reads aligned (%)
93.2
Duplicates removed (%)
10.5
Number of peaks
29230 (qval < 1E-05)

Base call quality data from DBCLS SRA