GSM2905784: GPS2 ChIP-seq in MALE WT BAT (brown adipose tissue); Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Adipocyte
Cell type
Brown adipocytes
NA
NA
Attributes by original data submitter
Sample
source_name
mouse brown adipose tissue (BAT)
background strain
129sv/C57BL6J
genotype/variation
GPS2 wildtype
gender
male
tissue
mouse brown adipose tissue (BAT)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tissue was minced with curved scissors (slurry like) and put in 10ml tubes containing PBS 1% formaldehyde for crosslink for 15 min at RT and neutralized with 0.125 M glycine. Tissue was then shredded in Lysis Buffer using a Bullet Blender Homogenizer at max power for 5 min 4°C. After sonication, chromatin was incubated with 2¬μg of antibody at 4 °C overnight. Immunoprecipitated complexes were collected using sepharose beads A (Life Technologies). After extensive washes the DNA was extracted and purified by phenol/chloroform extraction. The library construction protocol followed the manufacturer's instructions.