Antigen

Antigen Class

TFs and others

Antigen

CHD7

Cell type

Cell type Class

Pluripotent stem cell

Cell type

iPSC derived neural crests

NA

NA

Sample

source_name

hiPSC-derived neural crest cell

tissue

hiPSC-derived neural crest cell

genotype/variation

wild type

chip-antibody

CHD7 (CST, #6505, clone D3F5)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For single fixation with formaldehyde, cells were fixed with 1% formaldehyde-containing medium for 10 min. For double fixation, the cells were first fixed with 2 mM EGS for 30 min followed by with 1% formaldehyde-containing medium for 10 min. Libraries were prepared using KAPA Hyperprep Kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

31561272

Reads aligned (%)

98.5

Duplicates removed (%)

58.3

Number of peaks

3590 (qval < 1E-05)

hg19

Number of total reads

31561272

Reads aligned (%)

97.5

Duplicates removed (%)

59.9

Number of peaks

3657 (qval < 1E-05)