Antigen

Antigen Class

Histone

Antigen

H3K9me2

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

H3K9me2-ChIPSeq-K3AKO- SF

cell type

HCT116

genotype/variation

KDM3A knockout

culture condition

SF; addition of FBS into the no-serum culture after cell-starved for for 24 h

chip antibody

H3K9me2(abcam,ab1220)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated and enzyme digestion nuclei and histone-DNA complexes were isolated with antibody. Vazyme ND604 kit was used for ChIPSeq library construction. Brifely, about 10-100ng dsDNA was used as initiation for end repair and prepared for adaptor ligation. After adaptor ligation, PCR amplification with 8-13 cycles for each library and gel selection was used for library size selection and purification.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

23007880

Reads aligned (%)

89.2

Duplicates removed (%)

4.3

Number of peaks

543 (qval < 1E-05)

hg19

Number of total reads

23007880

Reads aligned (%)

88.1

Duplicates removed (%)

4.7

Number of peaks

314 (qval < 1E-05)