Antigen

Antigen Class

TFs and others

Antigen

Chd4

Cell type

Cell type Class

Embryo

Cell type

Embryonic heart

NA

NA

Sample

source_name

E10.0 cardiac tissue

strain

CD1

genotype

WT

development stage

Embryonic day 10.0

tissue

cardiac

chip antibody

Anti-CHD4 antibody [3F2/4] - ChIP Grade, Abcam, catalog# ab70469, mouse monoclonal

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Embryonic hearts were collected in PBS, fixed and DNA was isolated for ChIP-seq according to methods in [Wilczewski et. Al, 2017]. Libraries were prepared using the Nextera XT DNA Library Kit.

Sequencing Platform

instrument_model

NextSeq 500

mm10

Number of total reads

70672501

Reads aligned (%)

97.6

Duplicates removed (%)

39.7

Number of peaks

5039 (qval < 1E-05)

mm9

Number of total reads

70672501

Reads aligned (%)

97.4

Duplicates removed (%)

39.9

Number of peaks

5202 (qval < 1E-05)