Sample information curated by ChIP-Atlas

Antigen

Antigen Class
ATAC-Seq
Antigen
ATAC-Seq

Cell type

Cell type Class
Blood
Cell type
AML246
NA
NA

Attributes by original data submitter

Sample

source_name
AML246 ATACseq Dox addition/withdrawal
treatment
untreated
sort parameters
total viable cells

Sequenced DNA Library

library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Cells were isolated by FACS and ChIP was performed Libraries were prepared using the Nextera Kit from 50,000 cells per standard ATACseq protocols described in Buenrostro et al, Nature Methods 10:1213 (2013). Libraries were size selected from 200-700bps on a PippenPrep and sequenced on an Illumina NextSeq500 with 75bp PE reads.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
67917724
Reads aligned (%)
80.3
Duplicates removed (%)
35.5
Number of peaks
20945 (qval < 1E-05)

mm9

Number of total reads
67917724
Reads aligned (%)
80.2
Duplicates removed (%)
35.6
Number of peaks
20891 (qval < 1E-05)

Base call quality data from DBCLS SRA