Sample information curated by ChIP-Atlas

Antigen

Antigen Class
RNA polymerase
Antigen
RNA polymerase II

Cell type

Cell type Class
Prostate
Cell type
C4-2
Tissue
Prostate
Cell Type
Epithelial
Disease
Prostate Cancer

Attributes by original data submitter

Sample

source_name
Human prostate cancer cell line C42B
tissue
Human prostate cancer cell line C42B
cell line
C42B
chip antibody
POLR2A
sirna
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, cells were crosslinked using 1% formaldehyde andl lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq: Libraries were barcoded (NEXTflex™ DNA Barcodes) (Bio Scientific, Austin, TX).

Sequencing Platform

instrument_model
Illumina HiSeq 3000

hg38

Number of total reads
25394637
Reads aligned (%)
98.8
Duplicates removed (%)
22.1
Number of peaks
1181 (qval < 1E-05)

hg19

Number of total reads
25394637
Reads aligned (%)
97.8
Duplicates removed (%)
23.6
Number of peaks
1206 (qval < 1E-05)

Base call quality data from DBCLS SRA