GSM2907216: ChIPseq-GRKO abGFP NT rep1; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
GFP
Cell type
Cell type Class
Breast
Cell type
3617
NA
NA
Attributes by original data submitter
Sample
source_name
3617-KOGR cells, non-treated, GFP ChIP
cell line
3617-KOGR
cell type
mouse adenocarcinoma
stably expressing
NA
treatment
no treatment
concentration
NA
time
NA
chip antibody
GFP (Abcam, ab290, lot # GR278073-1)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
For ChIP-seq, lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated with specified antibody. For ATAC-seq, 100 000 isolated nuclei were treated with 2.5 µl Nextera Tn5 Transposase from Nextera kit. For RNA-seq, RNA was extracted using the PureLink RNA mini kit. Libraries were prepared for sequencing using standard Illumina protocols. For ChIP-seq, Illumina TruSeq Chip Sample Prep Kit; for ATAC-seq, Illumina Nextera DNA Library Prep Kit; for RNA-seq, Illumina Stranded Total RNA.