Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Blood

Cell type

Th2 Cells

MeSH Description

A subset of helper-inducer T-lymphocytes which synthesize and secrete the INTERLEUKINS IL-4; IL-5; IL-6; and IL-10. These cytokines influence B-cell development and antibody production as well as augmenting humoral responses.

Sample

source_name

Early Th2

strain/background

Bcl11b[fl/fl]-CreERT2 mice on C57BL/6 background

isolated cell type

Naive CD4 T cells

sorting condition

CD4+CD44high

culture condition

Early Th2

genotype/variation

Bcl11b KO

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

ChIP-Seq experiments were performed following iDeal ChIP-seq Kit for Histones (C01010051, Diagenode) using anti-H3K27ac (C15410196, Diagenode). DNase-Seq followed the protocols described previously in (PMID: 26605532). Library constructions ChIP-Seq and DNase-Seq follow the protocols described previously in (PMID: 19528076) and (PMID: 26605532).

Sequencing Platform

instrument_model

Illumina HiSeq 3000

mm10

Number of total reads

14517216

Reads aligned (%)

94.8

Duplicates removed (%)

20.2

Number of peaks

16737 (qval < 1E-05)

mm9

Number of total reads

14517216

Reads aligned (%)

94.7

Duplicates removed (%)

20.4

Number of peaks

16649 (qval < 1E-05)