Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Epidermis

Cell type

Keratinocytes

MeSH Description

Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Sample

source_name

mouse primary epidermal keratinocytes

strain

C57BL/6

age

newborn

tissue

skin

cell type

mouse primary epidermal keratinocytes

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cell were fixed with 1% formaldehyde in PBS, the nuclei were isolated, chromatin was sonicated and immuno-precipitated using anti-Brg1 anti-bodies (Santa Cruz Biotechnologies). The DNA has been isolated from the immuno-precipitated and input chromatin Indexed ChIP-seq libraries were generated using NEBNext reagents (New England Biolabs) and

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

72658772

Reads aligned (%)

96.8

Duplicates removed (%)

11.2

Number of peaks

2008 (qval < 1E-05)

mm9

Number of total reads

72658772

Reads aligned (%)

96.6

Duplicates removed (%)

11.1

Number of peaks

1896 (qval < 1E-05)