Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Gonad

Cell type

Testis

MeSH Description

The male gonad containing two functional parts: the SEMINIFEROUS TUBULES for the production and transport of male germ cells (SPERMATOGENESIS) and the interstitial compartment containing LEYDIG CELLS that produce ANDROGENS.

Sample

source_name

testes

strain

CD1

chip antibody

Rabbit anti-H3K4me3 (Millipore 07-473)

tissue

testes

agent

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

The testes from F1 generations were taken for anti-H3K4me3 ChIP-seq as previously described (Gely-Pernot et al, 2015). Two biological replicates used for each condition. We hypothesize that chlordecone cause changes in H3K4me3 due to transgenerational effect of pesticides (Skinner et al, Science 2005).

Sequencing Platform

instrument_model

Illumina HiSeq 4000

mm10

Number of total reads

121957674

Reads aligned (%)

96.8

Duplicates removed (%)

61.3

Number of peaks

45865 (qval < 1E-05)

mm9

Number of total reads

121957674

Reads aligned (%)

96.7

Duplicates removed (%)

61.4

Number of peaks

46719 (qval < 1E-05)