Antigen

Antigen Class

DNase-seq

Antigen

DNase-Seq

Cell type

Cell type Class

Kidney

Cell type

293

Primary Tissue

Kidney

Tissue Diagnosis

Normal

Sample

source_name

HEK293_DNase

cell line

HEK293

Sequenced DNA Library

library_strategy

DNase-Hypersensitivity

library_source

GENOMIC

library_selection

DNase

library_construction_protocol

DNase-seq was conducted on 50 million cells as previously described (Song and Crawford, 2010), with the minor modification of using 5' phosphorylated oligo 1b. Samples digested with 12U, 4U and 1.2U total DNase I were pooled.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

170437474

Reads aligned (%)

0.1

Duplicates removed (%)

31.1

Number of peaks

101 (qval < 1E-05)

hg19

Number of total reads

170437474

Reads aligned (%)

0.1

Duplicates removed (%)

31.4

Number of peaks

102 (qval < 1E-05)