GSM2898193: control LSK ATAC-seq replicate 1; Mus musculus; ATAC-seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
ATAC-Seq
Antigen
ATAC-Seq
Cell type
Cell type Class
Blood
Cell type
LSK cells
NA
NA
Attributes by original data submitter
Sample
source_name
LSK cells sorted from bone marrow
cell type
LSK cells
strain
C57BL/6
tissue
bone marrow
age
~3 month old
genotype
control
Sequenced DNA Library
library_strategy
ATAC-seq
library_source
GENOMIC
library_selection
other
library_construction_protocol
Total bone marrow cells were isolated from long bones (femur and tibia; 5 mice per experimental replicate) following by treatment with the ACK lysis buffer (0.15 M NH4Cl, 10 mM KHCO3, 0.1 mM EDTA [pH 7.2]) to remove red blood cells. An sorting of FACS-based analysis of lineage-/Sca1+/c-Kit+ (LSK) cells was carried out using a FACSAria II machine (UNC flow core) using the already defined protocol. ATAC-seq was carried out utilizing the Epicentre Tn5 transposome and Illuminia kit according to standard manufacturer's manuals.