Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
ILC2
NA
NA

Attributes by original data submitter

Sample

source_name
Innate type-2 lymphocytes
strain
C57BL/6
cell type/growth condition
ILC2s were sorted from the MLN of IL-25-treated mice
antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
~3 x 10^7 cells were harvested, crosslinked with formaldehyde, sonicated, IPed with appropriate antibodies and reverse-crosslinked to obtain 300-500 bp DNA fragments. Standard Illumina kit was used for library construction.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
61850649
Reads aligned (%)
99.4
Duplicates removed (%)
9.1
Number of peaks
290 (qval < 1E-05)

mm9

Number of total reads
61850649
Reads aligned (%)
99.3
Duplicates removed (%)
9.0
Number of peaks
294 (qval < 1E-05)

Base call quality data from DBCLS SRA